Document Type
Article
Publication Date
1-1-1995
Published In
Biophysical Journal
Abstract
We report the resonance light scattering (RLS) spectra of chlorophyll a aggregated in a 9:1 solution of formamide and pH 6.8 phosphate buffer. The aggregate formed after 2 h of mixing, referred to as Chl(469), shows a strong scattering feature at 469 nm (Soret band) and a much weaker feature at 699 nm (Q(y) band). A kinetic investigation confirmed that the aggregation process is cooperative, and also detected one intermediate (Chl(458)) with a strong RLS spectrum but only a weak CD spectrum. We propose that aggregation proceeds via at least three steps: 1) formation of a nucleating species, probably a dimer of chlorophylls; 2) formation of large aggregates with little or no secondary structure (e.g., Chl(458)); and 3) conformational change to form helical aggregate (Chl(469)).
Recommended Citation
J. C. de Paula, J. H. Robblee, and Robert F. Pasternack.
(1995).
"Aggregation Of Chlorophyll a Probed By Resonance Light Scattering Spectroscopy".
Biophysical Journal.
Volume 68,
Issue 1.
335-341.
DOI: 10.1016/s0006-3495(95)80192-x
https://works.swarthmore.edu/fac-chemistry/109
Comments
This work is freely available courtesy of the Biophysical Society and Elsevier.