Biological And Environmental Factors Affecting Ultrasound-Induced Hemolysis In Vitro: 2. Medium Dissolved Gas (pO2) Content
Ultrasound In Medicine And Biology
The data collected in this project supported the a priori hypothesis that the concentration of dissolved oxygen in whole human blood in vitro affected the extent of ultrasound (US)-induced hemolysis under conditions conducive to the occurrence of inertial cavitation. Aliquots of whole human blood in vitro with a relatively high O(2) level had statistically significantly more 1-MHz US-induced hemolysis than aliquots with a relatively low O(2) level in the presence of controlled gas nucleation (Albunex or ALX, supplementation), with US-induced hemolytic yields being substantially less at 2.2- and 3.5-MHz exposures or in the absence of ALX-supplementation at otherwise comparable acoustic pressures, pulse lengths and duty factors. Passive cavitation detection (pcd) measures indicated a linear relationship for hemolysis up to about 70% and pcd values (R(2) = 0.99).
M. W. Miller, E. Carr Everbach, W. M. Miller, and L. F. Battaglia.
"Biological And Environmental Factors Affecting Ultrasound-Induced Hemolysis In Vitro: 2. Medium Dissolved Gas (pO2) Content".
Ultrasound In Medicine And Biology.