Effects Of Acoustic Cavitation On Platelets In The Presence Of An Echo-Contrast Agent
Ultrasound In Medicine And Biology
A suspension of human platelets in autologous plasma or buffer solution with and without a microbubble echo-contrast agent was exposed in vitro to 730 W/cm2 (ISPPA) ultrasound pulses of duration 40-160 microseconds at 1 MHz and 20-Hz pulse repetition frequency. Inertial cavitation occurring within the samples was monitored during the exposures and a measure of average cavitational activity was calculated for each 5-min exposure. This quantity, with the other acoustic parameters, accounted for up to 75% of the variation in the destruction of platelets as measured by Coulter counter and 83.5% of the release of bound radiolabel using a multiple-interaction statistical model. When the echo-contrast agent was absent, negligible cavitation occurred and the amount of platelet destruction was statistically indistinguishable from sham (no-ultrasound) exposures. Therefore, microbubble echo-contrast agents may interact with ultrasound to cause platelet lysis through the mechanism of inertial cavitation.
E. Carr Everbach, I. R. S. Makin, C. W. Francis, and R. S. Meltzer.
"Effects Of Acoustic Cavitation On Platelets In The Presence Of An Echo-Contrast Agent".
Ultrasound In Medicine And Biology.