Expression And Purification Of Sperm Whale Myoglobin
Journal Of Chemical Education
We present a multiweek laboratory exercise that exposes students to the fundamental techniques of bacterial expression and protein purification through the preparation of sperm whale myoglobin. Myoglobin, a robust oxygen-binding protein, contains a single heme that gives the protein a reddish color, making it an ideal subject for the teaching laboratory as students can follow the progress of the protein visually in real time. The purification uses both ion-exchange and size-exclusion chromatography, thus, introducing students to two of the most common separation methods. Students evaluate the quality of the purification via sodium dodecyl sulfate−polyacrylamide gel electrophoresis. In most cases, this procedure produces sufficient quantities of pure protein for further laboratory exercises studying the properties of myoglobin.
Stephen T. Miller, Virginia M. Heck, and Caroline A. Burkhard.
"Expression And Purification Of Sperm Whale Myoglobin".
Journal Of Chemical Education.