Identification And Functional Analysis Of The Essential And Regulatory Light Chains Of The Only Type II Myosin Myo1p In Saccharomyces Cerevisiae

Authors

J. Luo
Elizabeth Ann Vallen, Swarthmore CollegeFollow
C. Dravis
S. E. Tcheperegine
B. Drees
E. BiFollow

Document Type

Article

Publication Date

6-21-2004

Published In

Journal Of Cell Biology

Abstract

Cytokinesis in Saccharomyces cerevisiae involves coordination between actomyosin ring contraction and septum formation and/or targeted membrane deposition. We show that Mlc1p, a light chain for Myo2p (type V myosin) and lqg1p (IQGAP), is the essential light chain for Myo1p, the only type 11 myosin in S. cerevisiae. However, disruption or reduction of Mlc1p-Myo1p interaction by deleting the Mlc1p binding site on Myo1p or by a point mutation in MLC1, mlc1-93, did not cause any obvious defect in cytokinesis. In contrast, a different point mutation, mlc1-11, displayed defects in cytokinesis and in interactions with Myo2p and lqg1p. These data suggest that the major function of the Mlc1p-Myo1p interaction is not to regulate Myo1p activity but that Mlc1p may interact with Myo1p, lqg1p, and Myo2p to coordinate actin ring formation and targeted membrane deposition during cytokinesis. We also identify Mlc2p as the regulatory light chain for Myo1p and demonstrate its role in Myo1p ring disassembly, a function likely conserved among eukaryotes.

Comments

This work is freely available courtesy of Rockefeller University Press.

Recommended Citation

J. Luo, Elizabeth Ann Vallen, C. Dravis, S. E. Tcheperegine, B. Drees, and E. Bi. (2004). "Identification And Functional Analysis Of The Essential And Regulatory Light Chains Of The Only Type II Myosin Myo1p In Saccharomyces Cerevisiae". Journal Of Cell Biology. Volume 165, Issue 6. 843-855. DOI: 10.1083/jcb.200401040
https://works.swarthmore.edu/fac-biology/8