Document Type
Article
Publication Date
4-1-2001
Published In
Journal Of Bacteriology
Abstract
Mitomycin C (MMC), a DNA-damaging agent, is a potent inducer of the bacterial SOS response; surprisingly, it has not been used to select resistant mutants from wild-type Escherichia coli. MMC resistance is caused by the presence of any of four distinct E. coli genes (mdfA, gyrl, rob, and sdiA) on high-copy-number vectors. mdfA encodes a membrane efflux pump whose overexpression results in broad-spectrum chemical resistance. The gyrI (also called sbmC) gene product inhibits DNA gyrase activity in vitro, while the rob protein appears to function in transcriptional activation of efflux pumps. SdiA is a transcriptional activator of ftsQAZ genes involved in cell division.
Recommended Citation
Y. Wei, Amy Cheng Vollmer, and R. A. LaRossa.
(2001).
"In Vivo Titration Of Mitomycin C Action By The Presence Of Four Escherichia Coli Genomic Regions On Multicopy Plasmid".
Journal Of Bacteriology.
Volume 183,
Issue 7.
2259-2264.
DOI: 10.1128/JB.183.7.2259–2264.2001
https://works.swarthmore.edu/fac-biology/15
Comments
This work is freely available courtesy of American Society for Microbiology.