Date of Award
© 2023 Erin V. Chen. This work is freely available courtesy of the author. It may be used under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) license. For all other uses, please contact the copyright holder.
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 4.0 International License.
Bachelor of Arts
Engineering, Chemistry & Biochemistry
E. Carr Everbach
Liliya A. Yatsunyk
G-quadruplex DNA structures are highly relevant subjects of biochemical study, implicated in human diseases and cancer. Conventional methods for G-quadruplex DNA structure separation are time-consuming, environmentally unfriendly, and low throughput. Thus, there is a pressing need for alternative methods of separation to be developed for G-quadruplex DNAs. Here we present work in designing a separation method using the capillary electrophoresis instrument. Capillary electrophoresis is a highly automated, reusable, and quantitative technique that separates analytes based on their size and charge. A capillary method for G-quadruplex separation would be a valuable analytical tool to supplement the biophysical methods available by addressing the pitfalls of conventional separation techniques. Through a series of informed experiments, we attempted to design a broadly applicable capillary electrophoresis method for G-quadruplex structural separation. Although a final method was not completed, we have engineered coupled experimental techniques to aid in the overall design process. Additionally, we demonstrated the presence of multiple structures within the capillary via non-symmetric peaks in the electropherogram.
Chen, Erin V. , '23, "Method Design for Separation of Non-Canonical DNA Structures via Capillary Electrophoresis" (2023). Senior Theses, Projects, and Awards. 280.