Extracellular matrix and associated proteins in Aiptasia pallida: Immunofluorescence detection and attempts to alter matrix assembly
Date of Award
© 2014 Valentina E. Garcia. All rights reserved. Access to this work is restricted to users within the Swarthmore College network and may only be used for non-commercial, educational, and research purposes. Sharing with users outside of the Swarthmore College network is expressly prohibited. For all other uses, including reproduction and distribution, please contact the copyright holder.
Bachelor of Arts
Elizabeth Ann Vallen
In many organisms the extracellular matrix (ECM) is involved in the migration of cells. This thesis investigates the role of the ECM in the Aiptasia-Symbiodinium symbiosis. Commercially available antibodies against mammalian collagen IV, laminin, and beta-integrin successfully label Aiptasia proteins in both symbiotic and aposymbiotic anemones. In an attempt to alter the ECM, anemones were incubated in ethyl-3,4- dihyroxybenzoate (EDHB) and beta-aminopropionitrile (BAPN). These anemones presented with distinct morphological differences, however, they did not show notable changes to the ECM as detected by immunofluorescence. EDHB does cause bleaching of symbiotic anemones, possibly by affecting the algal cell wall.
Garcia, Valentina E. , '14, "Extracellular matrix and associated proteins in Aiptasia pallida: Immunofluorescence detection and attempts to alter matrix assembly" (2014). Senior Theses, Projects, and Awards. 130.