Date of Award

Spring 2012

Document Type

Restricted Thesis

Terms of Use

© 2012 Rosalie E. Lawrence. All rights reserved. Access to this work is restricted to users within the Swarthmore College network and may only be used for non-commercial, educational, and research purposes. Sharing with users outside of the Swarthmore College network is expressly prohibited. For all other uses, including reproduction and distribution, please contact the copyright holder.

Degree Name

Bachelor of Arts

Department

Biology

First Advisor

Nicholas J. Kaplinsky

Abstract

Heat Shock Granules (HSGs) are subcellular structures composed of small Heat Shock Proteins (sHSPs) and misfolded proteins that form in response to heat stress in plants. While sHSPs are found in other organisms, HSGs have only been reported in plant cells and only in response to heat stress. This thesis examines the signaling pathways that regulate the transcription of sHSPs and the formation of HSGs and investigates whether heat is the only stress that could activate these pathways. By visualizing HSGs in an Arabidopsis thaliana BOBBERl:GFP reporter line using still and 4-D confocal microscopy, we characterize HSG formation and HSG structural qualities such as volume and shape. 4D tracking is used to describe dynamic behavior. We also show that inducing protein misfolding by treating live seedlings with amino acid analog L-Azetidine2-Carboxylic Acid (AZC) or proteasome inhibitor MG132 induces granule formation. We propose that the term "Heat Shock Granule" is a misnomer, since HSG formation can be catalyzed by misfolded protein stress in the absence of heat treatment.

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