Pegylation Of Magnetically Oriented Lipid Bilayers
Journal Of Magnetic Resonance
We report NMR data for magnetically oriented phospholipid bilayers which have been doped with a lipid derivatized with a polyethylene glycol polymer headgroup to stabilize samples against aggregation. C-13, P-31, and H-2 NMR data indicate that the incorporation of PEG2000-PE (1% molar to DMPC) does not interfere with the orientation properties of bicelles prepared at 25% w/v with or without the presence of lanthanide. Bicelles prepared at 10% w/v are also shown to orient when PEG2000-PE is added. The addition of PEG2000-PE to cholesterol-containing, lanthanide-flipped bicelles is shown to inhibit sample phase separation and improve spectral quality. Furthermore, the addition of PEG2000-PE to high w/v bicelles (40% w/v) is demonstrated to lead to an increase in overall sample order. (C) 2000 Academic Press.
V. King, M. Parker, and Kathleen P. Howard.
"Pegylation Of Magnetically Oriented Lipid Bilayers".
Journal Of Magnetic Resonance.