N-Methyl Mesoporphorin IX, A Reporter On GQ-DNA, Forms A Dimer In Aqueous Solution
Document Type
Poster Session
Publication Date
2-8-2024
Published In
Biophysical Journal
Abstract
N-methyl mesoporphyrin IX (NMM) has rapidly become a significant tool for the characterization of quadruplex DNA (GQ-DNA) structures. These novel DNA structures are of interest due to their enrichment in protooncogene sequences and telomeres [Kosiol 2021]. As NMM becomes a standard tool in the sub-field of GQ-DNA research, it becomes increasingly important to understand its behavior. NMM's utility is based on a shift in absorbance from a max at 379 nm to a max at 400 nm in the presence of GQ binding partners, and fluorescence enhancement upon the presence of binding partners [Yett 2019]. In this work we present data showing that NMM, in aqueous solution, without GQ-DNA present, shows a concentration dependent shift in peak absorbance from 400 nm to 379 nm with increasing concentration. Analysis, using singular value decomposition (SVD), shows that this shift is consistent with NMM forming a dimer. In addition, NMM displays increasing fluorescence intensity with increasing temperature, consistent with fluorescence quenching in the dimer state. These data suggest that some of the attributes of NMM as a GQ-DNA probe may be due to the presence of GQ-DNA, and associated binding, changing the effective concentration of free NMM in solution.
Conference
Biophysical Society Annual Meeting 2024
Conference Dates
February 10-14, 2024
Conference Location
Philadelphia, PA
Recommended Citation
D. E. Farrell, Liliya A. Yatsunyk, and S. H. Pfeil.
(2024).
"N-Methyl Mesoporphorin IX, A Reporter On GQ-DNA, Forms A Dimer In Aqueous Solution".
Biophysical Journal.
Volume 123,
Issue 3, suppl. 1.
496a-496a.
DOI: 10.1016/j.bpj.2023.11.3003
https://works.swarthmore.edu/fac-chemistry/295