Title

Drug Binding By Branched DNA: Selective Interaction Of Tetrapyridyl Porphyrins With An Immobile Junction

Document Type

Article

Publication Date

2-13-1990

Published In

Biochemistry

Abstract

The differential binding of a number of water-soluble cationic porphyrins to a branched DNA molecule is reported. Tetrakis(4-N-methylpyridiniumy1)porphine (H₂TMpyP-4) interacts near the branch point with an immobile DNA junction formed from four 16-mer strands. Its Cu(I1) and Ni(I1) derivatives show stronger preferential binding in the neighborhood of the branch point. Axially liganded derivatives, Zn, Co, and Mn, also interact near this branch point, but in a different way. We use the reagents methidiumpropyl-EDTAoFe(I1) [MPE.Fe(II)] and bis(o-phenanthroline)copper(I) [(OP)₂,Cu(I)] to cleave complexes of DNA duplex controls and the junction with these porphyrins. The resulting cleavage patterns are consistent with previous evidence that the branch point provides a strong site for intercalative binding agents, which is not available in unbranched duplexes of identical sequence. The preferential scission by (OP)₂Cu(I) in the presence of Ni and Cu porphyrins near the branch point exceeds that seen for any agents we have studied. This hyperreactivity is not seen in the case of porphyrins with axial ligands, ZnTMpyP-4, CoTMpyP-4, and MnTMpyP-4, although these also interact near the branch point. The Zn derivative tends to protect sites close to the branch point from cutting, while the Co and Mn porphyrins moderately enhance cleavage of sites in this region.

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