Document Type
Article
Publication Date
10-12-2013
Published In
BMC Plant Biology
Abstract
Background: High temperature stress responses are vital for plant survival. The mechanisms that plants use to sense high temperatures are only partially understood and involve multiple sensing and signaling pathways. Here we describe the development of the RootScope, an automated microscopy system for quantitating heat shock responses in plant roots.Results: The promoter of Hsp17.6 was used to build a Hsp17.6(p):GFP transcriptional reporter that is induced by heat shock in Arabidopsis. An automated fluorescence microscopy system which enables multiple roots to be imaged in rapid succession was used to quantitate Hsp17.6p: GFP response dynamics. Hsp17.6(p):GFP signal increased with temperature increases from 28 degrees C to 37 degrees C. At 40 degrees C the kinetics and localization of the response are markedly different from those at 37 degrees C. This suggests that different mechanisms mediate heat shock responses above and below 37 degrees C. Finally, we demonstrate that Hsp17.6(p):GFP expression exhibits wave like dynamics in growing roots.Conclusions: The RootScope system is a simple and powerful platform for investigating the heat shock response in plants.
Keywords
Heat shock, Thermometer, Thermostat, Automated imaging, Quantitative imaging, Temperature, Arabidopsis
Recommended Citation
Erin J. Kast , '15; Minh-Duyen T. Nguyen , '13; Rosalie E. Lawrence , '12; C. Rabeler; and Nicholas J. Kaplinsky.
(2013).
"The RootScope: A Simple High-Throughput Screening System For Quantitating Gene Expression Dynamics In Plant Roots".
BMC Plant Biology.
Volume 13,
DOI: 10.1186/1471-2229-13-158
https://works.swarthmore.edu/fac-biology/119
Comments
This work is licensed under a Creative Commons Attribution 2.0 License.
This work is freely available under a Creative Commons license.